RFP Selector

A, Pull-down of mCherry from a mixture of GFP, mCherry and mTagBFP.

B, Immunoprecipitation of mCherry (•) from HeLa lysate.

  • In/Ft: 1/1000 of input and non-bound material.
  • E: Eluate from 1 µl of beads

C, Control experiment using functionalized beads lacking sdAbs.

Left panel: Immunoprecipitation of mCherry (•) from HeLa lysate.

  • In/Ft: 1/1000 of input and non-bound material, respectively;
  • E: Eluate from 1 µl of beads;

Right panel, Control experiment using functionalized beads lacking sdAbs.

Pull-down of fluorescent proteins from a mixture of GFP, mCherry and mTagBFP using Selector Resins

* Specific maturation band from RFP family members (see e.g. Gross et al. PNAS (2000) 11990-11995)

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Description

TagFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. TagFP Selector thus features high affinity and superior capacity for TagFP fusion proteins while showing negligible non-specific background. TagFP Selector is compatible not only with physiological buffers but also with high stringency buffers (see Product Data Sheet). TagFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

  • Specificity: Recognizes mTagBFP, mTagRFP, mTagRFP657 and mKate. Reactivity against other TagRFP derivatives not analyzed. Does not cross-react with common GFP- or dsRed/RFP derivatives including EBFP. For more detailed information see Product Specificity Chart below.
  • Support: 4% cross-linked agarose beads
  • Capacity: > 4 µg BFP per µl of packed beads (= 2µl of slurry)
  • Formulation: 50% slurry in 20% ethanol / PBS
  • Recommended for: Immunoprecipitation, Mass Spectrometry
  • TagFP Selector Kit: Our TagFP Selector Kit (Cat. No. N0510-Kit) contains 500 µl TagFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 20 MiniSpin columns.

Product List

PRODUCT NAMERESINAMOUNTCATALOG NUMBERORDER AT
TagFP Selector4% Agarose CL200 µl slurry for up to 10 reactionsN0510-SBuy online
TagFP Selector4% Agarose CL500 µl slurry for up to 25 reactionsN0510-MBuy online
TagFP Selector4% Agarose CL2000 µl slurry for up to 100 reactionsN0510-LBuy online
Selector Control4% Agarose CL400 µl slurry for up to 20 reactionsN0010-SBuy online

This information is provided without liability and may be subject to change without prior notification.