GST Selector

Immunoprecipitation of GST (•) from HeLa lysate.

  • In/Ft: 1/1000 of input and non-bound material.
  • E: Eluate from 1 µl of beads
  • Right panel: Control experiment using functionalized beads lacking sdAbs.
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GST Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GST Selector thus features high affinity and superior capacity for GST fusion proteins while showing negligible non-specific background. GST Selector is compatible not only with physiological buffers but also with high stringency buffers (see Product Data Sheet). GST Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

  • Specificity: Recognizes Schistosoma japonicum Glutathione S-transferase (GST)
  • Support: 4% cross-linked agarose beads
  • Capacity: > 4 µg GST per µl of packed beads (i.e. 2 µl of slurry)
  • Formulation: 50% slurry in 20% ethanol / PBS
  • Recommended for: Immunoprecipitation, Mass Spectrometry
  • GST Selector Kit: Our GST Selector Kit (Cat. No. N0210-Kit) contains 500 µl GST Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 20 MiniSpin columns.

Relevant Documents

Product List

GST Selector4% Agarose CL200 µl slurry for up to 10 reactionsN0210-SBuy online
GST Selector4% Agarose CL500 µl slurry for up to 25 reactionsN0210-MBuy online
GST Selector4%2000 µl slurry for up to 100 reactionsN0210-LBuy online
Selector Control4% Agarose CL400 µl slurry for up to 20 reactionsN0010-SBuy online