Description

GFP Selector is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4% cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no “leakage” of light and heavy chains from IgGs is observed during elution with SDS sample buffer. GFP Selector thus features high affinity and superior capacity for GFP fusion proteins while showing negligible non-specific background. GFP Selector immobilizes a wide range of GFP derivatives. For details see our Product Specificity Chart below. GFP Selector is compatible not only with physiological buffers but also with high stringency buffers (see Product Data Sheet). GFP Selector thus provides great freedom to adjust the binding and washing conditions to the experimental needs.

• Specificity: Recognizes GFP, mEGFP, superfolder GFP, Emerald GFP and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see Product Specificity Chart below.
• Capacity: > 4 µg GFP per µl of packed beads (i.e. 2 µl of slurry)
• Support: 4% cross-linked agarose beads
• Formulation: 50% slurry in 20% ethanol / PBS
• Recommended for: Immunoprecipitation, Mass Spectrometry
• GFP Selector Kit: Our GFP Selector Kit (Cat. No. N0310-Kit) contains 500 µl GFP Selector (sufficient for 25 reactions), 400 µl Selector Control (sufficient for 20 reactions) and 20 MiniSpin columns.