FluoTag®-X4 anti-GFP

HeLa cells transfected with the nuclear pore complex marker GFP-Nup98 were PFA-fixed and stained with FluoTag-X4 anti-GFP AbberiorStar635P. Shown is an overlay of the FluoTag-X4 anti-GFP signal (red) with DAPI.

Confocal images of HeLa cells transfected with the nuclear pore complex marker GFP-Nup98 stained with FluoTag-X4 anti-GFP AbberiorStar635P. A: GFP; B: FluoTag-X4 anti-GFP; C: Overlay of all channels including DAPI as nuclear marker.

FluoTags anti GFP coupled to Atto647N. Ratios of FluoTag/GFP intensities per cell (HeLa) normalized against FluoTag-Q/GFP ratio.

3T3 cells expressing GFP-Tubulin (A, green) were fixed with methanol and stained with FluoTag-X4 anti-GFP AbberiorStar580 (B, red). An overlay is shown in C together with the DAPI stain (blue) to show the position of nuclei.

HeLa cells expressing the nuclear pore marker Pom121-mCherry and GFP-Tubulin were PFA-fixed and stained with FluoTag-X4 anti-RFP AbberiorStar580 (red) and FluoTag-X4 anti-GFP AbberiorStar635P (green). Shown is an overlay with a DAPI stain (nuclei, blue).

HeLa cells transfected with the nuclear pore complex marker GFP-Nup98 stained with FluoTag-X4 anti-GFP AbberiorStar635P. A: Overlay of GFP and DAPI signals; B: Overlay of FluoTag-X4 anti-GFP and DAPI signals; C: Overlay of all channels.

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Description

FluoTag®-X4 anti-GFP is a blend of two in-house developed single-domain antibodies (sdAbs) that recognize two distinct epitopes on GFP and its most common derivatives with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart below. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features – superior brightness and precise fluorophore placement – render our FluoTag-X4 products excellent tools for all microscopy techniques.

  • Antigen: GFP
  • Specificity: Recognizes native GFP, mEGFP, superfolder GFP, Emerald and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see the Product Reactivity Chart below.
  • Produced in: E.coli
  • Concentration: 2.5 µM protein, 5 µM fluorophore
  • Recommended dilution for IF/ ICC: 1:250
  • Available fluorohores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.

Product List

PRODUCT NAMEVOLUMECATALOG NUMBERORDER AT
FluoTag-X4 anti GFP20 µlN0304Buy online
FluoTag-X4 anti GFP200 µlN0304Buy online

This information is provided without liability and may be subject to change without prior notification.

References

  • Hussain et al. (2018) Inhibition of oxidative stress in cholinergic projection neurons fully rescues aging-associated olfactory circuit degeneration in Drosophila. Elife. 2018 Jan 18;7
    PMID: 29345616 DOI: 10.7554/eLife.32018
  • Fulterer et al. (2018) Active Zone Scaffold Protein Ratios Tune Functional Diversity across Brain Synapses
    DOI:https://doi.org/10.1016/j.celrep.2018.03.126

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