FluoTag®-X2 anti-TagFP

3T3 cells transfected with the mitochondrial marker TOM70-mTagBFP, stained with FluoTag-X2 anti-TagFP Atto542 (red) and DAPI (blue). Due to the weak expression of the mTagBFP marker, only the DAPI signal is visible in the blue channel.

3T3 cells transfected with the mitochondrial marker TOM70-mTagBFP (A, false color illustration in red), stained with FluoTag-X2 anti-TagFP Atto488 (B, green). An overlay of both channels in shown in C.

3T3 cells expressing plasma membrane-anchored mTagBFP and mCherry-Tubulin (PFA-fixed) stained with FluoTag-X2 anti-TagFP Atto488 (A, green) and FluoTag-X4 anti-RFP AbberiorStar635P (B, red). An overlay is shown in C together with the BFP/DAPI channel (blue).

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FluoTag®-X2 anti-TagFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes fluorescent proteins deriving from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657 and mKate with high affinity and specificity. For a complete specificity chart see the Product Specificity Chart below. Our FluoTag-X2 series features two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X2 series can thus improve the localization accuracy by 10-15 nm. Both features – enhanced brightness and precise fluorophore placement – render our FluoTag-X2 products superior tools for all microscopy techniques.

  • Specificity: Recognizes mTagBFP, mTagRFP and mKate and most common fluorescent proteins deriving from Entacmaea quadricolor. Does not cross-react with common GFP- or dsRed derivatives.
  • Produced in: E.coli
  • Concentration: 2.5 µM protein, 5 µM fluorophore
  • Recommended dilution for IF/ ICC: 1:500
  • Available fluorophores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.

Product List

FluoTag-X2 anti-TagFP20 µlN0502Buy online
FluoTag-X2 anti-TagFP200 µlN0502Buy online

This information is provided without liability and may be subject to change without prior notification.