FluoTag®-X2 anti-Rabbit IgG

3T3 cells decorated with the rabbit anti-beta-Actin (SYSY cat. no. 251 003) were stained with FluoTag-X2 anti-Rabbit Atto 565 with additional DAPI stain.

IHC on PFA fixed rat brain slices. Primary rabbit anti-GFAP (SYSY cat no. 173 002) decorated with FluoTag X2 anti-Rabbit Atto 565 (false color representation in cyan) and primary guinea pig anti-Parvalbumin (SYSY cat no. 195 004) decorated with FluoTag X2 anti-guinea pig AbberiorSTAR 635P (false color representation in yellow). Additionally a DAPI stain was performed.

PFA fixed primary neurons decorated with rabbit anti-Vglut1 (SYSY cat. no. 135 303) and stained with FluoTag anti-Rabbit AbberiorSTAR 580 (red). Co-staining of Map2 (green) and dapi (blue).

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FluoTag®-X2 anti-Rabbit is an isotype- and species-specific FluoTag® directed against immunoglobulins from Rabbit. In our FluoTag-X2 series, two fluorophore molecules are site-specifically coupled to one individual FluoTag molecule. The reagent therefore targets four fluorophores to your primary Rabbit antibody. Due to the monovalent binding, there are no primary and secondary antibody clusters formed, leading to better epitope accessibility and a more precise localization. Since our FluoTags are recombinantly expressed, there is no batch-to-batch variation.

  • Antigen: Rabbit IgG
  • Specificity: Recognizes native Rabbit IgG. It does not cross-react with Mouse, Guinea Pig, Chicken, Rat, Donkey, Goat, Pig, Horse, Cow or Human IgGs.
  • Produced in: E. coli
  • Concentration: 2.5 µM protein, 5 µM fluorophore
  • Recommended dilution for IF/ICC/IHC: 1:500
  • Available fluorophores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.

Product List

FluoTag®-X2 anti-Rabbit IgG500 µLN1002Buy online
FluoTag®-X2 anti-Rabbit IgG2 mL (4 x 500 µL)N1002Buy online

This information is provided without liability and may be subject to change without prior notification.