FluoTag®-Q anti-TagFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes fluorescent proteins deriving from Entacmaea quadricolor, such as mTagBFP, mTagRFP, mTagRFP657 and mKate with high affinity and specificityFor a complete specificity chart see the Product Specificity Chart below. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
- Specificity: Recognizes mTagBFP, mTagRFP and mKate and most common fluorescent proteins deriving from Entacmaea quadricolor. Does not cross-react with common GFP- or dsRed derivatives.
Produced in: E.coli
- Concentration: 5 µM protein, 5 µM fluorophore
- Recommended dilution for IF/ ICC: 1:1000
- Available fluorophores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.