FluoTag®-Q anti-GFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes GFP and its most common derivatives with high affinity and specificity. For a complete specificity chart see the Product Reactivity Chart below. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
- Antigen: GFP
- Specificity: Recognizes native GFP, mEGFP, superfolder GFP, Emerald and most common CFP and YFP variants. It does not cross-react with mCherry, mRFP, dsRed, mTagBFP or their most common derivatives. For more detailed information see the Product Reactivity Chart below.
- Produced in: E. coli
- Concentration: 5 µM protein, 5 µM fluorophore
- Recommended dilution for IF/ ICC: 1:1000
- Available fluorophores: Abberior® Star 488, Abberior® Star RED, Abberior® Star 580, Abberior® Star 635P, Atto 488, Atto 542, Atto 580, Atto 647N, Sulfo-Cyanin 3, Sulfo-Cyanin 5. For other fluorophores please contact us.